Autotolerant ceruloplasmin based biocathodes for implanted biological power sources

• Ceruloplasmin hemocompatibility was confirmed by calorimetry and IL-6 ELISA. Partial purification of ceruloplasmin from human serum gave enzyme higher activity. Direct mediated electron transfer bioelectroreduction oxygen achieved. Simplified methodology to evaluate the bioelectrode inflammatory response shown. High-performance autotolerant bioelectrodes should be ideally suited design implantable bioelectronic devices. Because its high redox potential ability reduce directly water, ceruloplasmin, HCp, only blue multicopper oxidase present in plasma, appears ultimate biocatalyst for biosensors also biocathodes biological power sources. In comparison fungal plant oxidases, e.g. Myrothecium verrucaria bilirubin Rhus vernicifera laccase, respectively, HCp blood is significantly reduced. allowed preserve native conformation biocatalytic Therefore, electrochemical studies were carried out with partially purified immobilised on nanostructured graphite electrodes at physiological pH temperature. Amperometric investigations revealed low reductive current densities, i.e . about 1.65 µA cm −2 oxygenated electrolyte absence any mediator, demonstrating nevertheless direct based O 2 first time. The density obtained system 12 Even though diminished blood, inadequate bioelectrocatalytic performance hinders use as a cathodic bioelement biofuel cell.